TSC AGAR BASE (500 gr)

For the detection and enumeration of Clostridium perfringens

Applications
Detection / Isolation / Enumeration
Isolation CLOSTRIDIUM PERFRINGENS
Packaging
Suspend 42 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 44-47ºC and aseptically add two vials of the selective supplement Clostridium perfringens (Cat. 6020) reconstituted in 5 ml of sterile distilled water. If desired, 25 ml of Egg Yolk Emulsion (Cat. 5152) can be added (Not indicated in ISO 7937). Homogenize gently and dispense into Petri dishes.

Clostridium perfringens supplement (Cat. 6020)

(1 vial for 500 ml of the medium)

D-Cycloserine ………………………200 mg



Formula
Casein Peptone 15
Soy Peptone 5
Yeast Extract 5
Disodium Disulfite (Anhydrous) 1
Ferric Ammonium Citrate 1
Bacteriological Agar 15