TETRATHIONATE BROTH BASE (MUELLER KAUFFMANN) (500 gr)

For the selective enrichment of Salmonella species from foods, water, feces,urine and other materials of sanitary interest



Applications
Selective enrichment
SALMONELLA
Packaging
Suspend 46 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. AVOID OVERHEATING. DO NOT AUTOCLAVE. Cool to 45-50ºC and aseptically add 20 ml per liter of an iodine solution to the medium on the same day of its use. Homogenize gently and dispense into sterile containers. Prepare the solution iodine-iodide by dissolving 6 g of Iodine crystals and 5 g of potassium iodide in 20 ml of distilled water. Dispense 10 ml into tubes, continually swirling the flask to maintain homogeneity.

Formula
Sodium Thiosulfate 30
Calcium Carbonate 10
Peptone Mixture 5
Bile Salts 1