REINFORCED CLOSTRIDIAL MEDIUM (European Pharmacopeia) (500 gr)

For the cultivation and enumeration of Clostridium and other anaerobes

Applications
Clostridium
ISOLATION AND IDENTIFICATION
Packaging
Suspend 38 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until comple dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50°C and, if desired,add 0.02 g/l of Polymyxin B in a sterile filtered solution. Distribute into appropriate containers.

Formula
Beef Extract 10
Peptone 10
Glucose Monohydrate 5
Sodium Chloride 5
Yeast Extract 3
Sodium Acetate 3
Soluble Starch 1
L-Cysteine Hydrochloride 0.5
Bacteriological Agar 0.5