RAKA - RAY AGAR BASE (500 gr)

Selective medium for the isolation of lactic acid bacteria in beer and beer fermentation



Applications
Beer fermentation process
OTHER APPLICATIONS
Packaging
Suspend 77.2 grams of the medium in one liter of distilled water. Add 10 ml of Sorbitan Monooleate. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. DO NOT OVEARTHEAT. Cool to 45-50ºC and aseptically add 3 gramsof Phenylethanol. Mix well and dispense into plates.

Formula
Tryptone 20
Maltose 10
Yeast Extract 5
Fructose 5
Glucose 5
Potassium Aspartate 2.5
Potassium Glutamate 2.5
Betaine Hydrochloride 2
Diammonium Hydrogen Citrate 2
Magnesium Sulfate 7H2O 2
Potassium Phosphate 2
Liver Extract 1
Manganese Sulfate 4H2O 0.66
N-Acetylglucosamine 0.5
Cycloheximide 0.007
Bacteriological Agar 17