EOSIN METHYLENE BLUE AGAR (E.M.B.) (500 gr)

For the isolation, cultivation and differentiation of gram negative enteric bacilli from clinical and other specimens



Applications
Isolation / Cultivation / Differentiation
COLIFORMS
ENTEROBACTERIA
Packaging
Suspend 36 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC, mix well, avoiding the formation of bubbles and dispense carefully into Petri Dishes. DO NOT OVEARHEAT. The prepared medium should be stored at 8-15°C. The color is tournasol blue. Sterilization reduces the methylene blue, leaving the medium orange in color. The normal purple may be restored by gently mixing. The reduced medium should be shaken to oxidize the methylene blue; otherwise a dark zone from the top extending downwards will gradually appear.

Formula
Bacteriological Peptone 10
Lactose 5
Sucrose 5
Dipotassium Phosphate 2
Eosin Y 0.4
Methylene Blue 0.065
Bacteriological Agar 13.5