COLUMBIA AGAR BASE (European Pharmacopeia) (500 gr)

For the isolation and cultivation of fastidious microorganisms and the determination of hemolytic reactions

Applications
Hemolytic Activity
Media for general use
OTHER APPLICATIONS
Packaging
Suspend 42.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121°C for 15 minutes. The medium is generally enriched with sterile defibrinated blood (sheep, rabbit or horse), serum or some other material. Cool to 45-50ºC and aseptically add 5-10% sterile defibrinated blood, homogenize gently and pour into Petri dishes. Be careful to avoid bubble formation when adding the blood.



Formula
Pancreatic Digest of Casein 10
Yeast Extract 5
Meat Peptic Digest 5
Sodium Chloride 5
Heart Pancreatic Digest 3
Maize Starch 1
Bacteriological Agar 13.5