ALKALINE PEPTONE WATER (500 gr)

For the detection of Alicyclobacillus in fruit juices

Applications
ALICYCLOBACILLUS
Packaging
Suspend 29 grams of the medium in one liter of distilled water .Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. Note.- The medium is manufactured with a pH of 5.3 ± 0.2 in order to mantain the gel strenght during sterilization. Adjust the pH at 4.0 ± 0.2 after autoclaving, adding 1.7 ml per liter of medium of 1 N H2SO4. Homogenize gently and pour intro petri plates.

Formula
D (+) glucose 5
Dihygrogenfosfate Potasium 3
Yeast Extract 2
Magnesium Sulfate 0.5
Calcium Chloride 0.25
Ammonium Sulfate 0.2
Calcium Chlorid 0.00066
Sodium Molibdate 0.0003
Zinc Sulfate 0.00018
Manganase Sulfate 0.00015
Boric Acid 0.0001
Bacteriological Agar 18