AGAR TEST PH 8,0 (500 gr)

Standard medium for the detection of antimicrobial inhibitors in meat and organ samples with Bacillus subtilis and Micrococcus luteus spores as test organisms

Applications
Antibiotic Assay
OTHER APPLICATIONS
Packaging
Suspend 27.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add a spore suspension of Bacillus subtilis (BGA) containing approximately 1x10^7 cfu/ml (final concentration in mediim 1x10^4 cfu/ml). If desired, add a suspension of Micrococcus luteus ATCC 9341 suspension. Pour into plates. The prepared medium should be used immediately.

Formula
Sodium Chloride 5.1
Casein Peptone 3.45
Meat Peptone 3.45
Trisodium Phosphate 12-hydrate 2.4
Bacteriological Agar 13