RM BASE AGAR MEDIUM (500 gr)

Solid medium for the maintenance and propagation of the promoter PL in the E.coli strains GI724, GI826 and GI698

Packaging
Suspend 45.6 grams of the dehydrated medium in 900 ml of distilled water, add 20 ml of 50% glycerol and adjust to a final volume of 1000 ml. Mix well. Heat with frequent agitation until complete dissolution. Sterilize in the autoclave at 121°C for 15 minutes. Add 1 ml of 100 µg/ml of ampiciline under sterile conditions and mix well. Cool to 45 - 50°C and pour into Petri dishes. Store at 4°C. The color of the prepared medium is amber.

Formula
Casaminoacids 20
Disodium Phosphate 6
Monopotassium Phosphate 3
Ammonium Chloride 1
Sodium Chloride 0.5
Magnesium Chloride 0.095
Bacteriological Agar 15