Native Sample Buffer for Protein Gels, (30 ml)

Use Native Sample Buffer to retain native protein structure and mass-to-charge ratios during protein electrophoresis. This sample buffer is nondenaturing, containing no SDS, and has no reducing agent. The mobility of proteins in native gels depends on a number of factors in addition to molecular weight, including protein shape and charge.

The biological activity of most proteins is retained
Some protein-protein interactions may be maintained
Native gel electrophoresis cannot be used for molecular weight estimation
Since migration is influenced by structure, proteins of the same size can often be easily resolved
Dry samples can be dissolved directly into the sample buffer
Precast gels that can be used for native electrophoresis
Precast GelsSizeGel Chemistry
Mini-PROTEAN® TGX™MiniLaemmli-like, long shelf life, fast separation with high resolution
Criterion™ TGX™MidiLaemmli-like, long shelf life, fast separation with high resolution
Mini-PROTEAN® TGX Stain-Free™MiniLaemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection
Criterion™ TGX Stain-Free™MidiLaemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection
Criterion™ Tris-HClMidiStandard Laemmli
Criterion Stain Free™ Tris-HClMidiStandard Laemmli, unique trihalo compounds for rapid fluorescent protein detection
Criterion XT Tris-AcetateMidiDiscontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins