10x Tris/Glycine Buffer for Western Blots and Native Gels (1 pz)

Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.
For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol
For tank blotting of native gels, without methanol
As a running buffer for native gels
Precast gels that can be used for native electrophoresis
Precast Gel TypeSizeGel Chemistry
Mini-PROTEAN® TGX™MiniLaemmli-like, long shelf life, fast separation with high resolution
Criterion™ TGX™Midi
Mini-PROTEAN® TGX Stain-Free™MiniLaemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection
Criterion™ TGX Stain-Free™Midi
Criterion™ Tris-HClMidiStandard Laemmli
Criterion Stain Free™ Tris-HClMidiStandard Laemmli, unique trihalo compounds for rapid fluorescent protein detection
Criterion XT Tris-AcetateMidiDiscontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins
Note: Most proteins have an acidic or slightly basic pI (~3–8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl >8.5) of interest are being separated, change the polarity of the electrodes, since they have a net positive charge.

Packaging Options
10x Buffer VolumeCatalog Number
1 L1610734
5 L cube1610771