ALE I (500 U)

Descrizione

CACNN/NNGTG
GTGNN/NNCAC

Source:
An E. coli strain that carries the cloned AleI gene Aureobacterium liquefaciens (S.K. Degtyarev)

Reagents Supplied:
NEBuffer 4


Enzyme Properties


Activity in NEBuffers:
NEBuffer 1:10%
NEBuffer 2:25%
NEBuffer 3:10%
NEBuffer 4:100%

When using a buffer other than the optimal (supplied) NEBuffer, it may be necessary to add more enzyme to achieve complete digestion.

Methylation Sensitivity:
dam methylation: Not sensitive
dcm methylation: Not sensitive
CpG methylation: Impaired by some combinations of overlapping

Heat Inactivation:
65°C for 20 minutes

Survival in a Reaction:
(+ +) Intermediate activity. Suitable for extended digestion, but < 8 hours.


Reaction & Storage Conditions


Reaction Conditions:
1X NEBuffer 4
Incubate at 37°C.

1X NEBuffer 4:
20 mM Tris-acetate
50 mM potassium acetate
10 mM Magnesium Acetate
1 mM Dithiothreitol
pH 7.9 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 µg of ? DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Concentration:
10,000 units/ml

Unit Assay Substrate:
? DNA

Storage Conditions:
20 mM Tris-HCl
300 mM NaCl
1 mM Dithiothreitol
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Storage Temperature:
-70°C

Diluent Compatibility:
Diluent A