AGE I (300 U)

Descrizione

A/CCGGT
TGGCC/A

Source:
An E. coli strain that carries the AgeI gene from Ruegeria gelatinovora (ATCC 25655).

Reagents Supplied:
NEBuffer 1 (10X)


Enzyme Properties


Activity in NEBuffers:
NEBuffer 1:100%
NEBuffer 2:50%
NEBuffer 3:10%
NEBuffer 4:75%

When using a buffer other than the optimal (supplied) NEBuffer, it may be necessary to add more enzyme to achieve complete digestion.

Methylation Sensitivity:
dam methylation: Not sensitive
dcm methylation: Not sensitive
CpG methylation: Blocked

Heat Inactivation:
65°C for 20 minutes

Survival in a Reaction:
(+) Intermediate activity. Suitable for extended digestion, but < 8 hours.


Reaction & Storage Conditions


Reaction Conditions:
1X NEBuffer 1
Incubate at 37°C.

1X NEBuffer 1:
10 mM Bis-Tris-Propane-HCl
10 mM MgCl2
1 mM Dithiothreitol
pH 7.0 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 µg of ? DNA in 1 hour at 37°C in a total reaction of 50 µl.

Concentration:
5,000 units/ml

Unit Assay Substrate:
? DNA

Storage Conditions:
10 mM Tris-HCl
250 mM NaCl
1 mM Dithiothreitol
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
0.15% Triton X-100
pH 7.4 @ 25°C

Storage Temperature:
-20°C

Diluent Compatibility:
Diluent C