BGREEN HotTaq EvaGreen qPCR Universal Mix (1 mL, 250 rxn)

Universal qPCR Mix for either ROX or no-ROX qPCR cyclers.
BGTAQ HF/HT HotTaq EvaGreen® qPCR Universal Mix is optimized for real-time quantitative PCR assays.
The ready-to-use mix includes BGTAQ HF/HT HotTaq DNA polymerase and other components necessary to perform the reaction – only water, template and primers needs to be added.
BGTAQ HF/HT HotTaq DNA polymerase is activated by a 12 min incubation step at 95°C.
This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

APPLICATIONS:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

ADVANTAGES:
- Specific and reproducible real time PCR
- Highly efficient in case of low copy number targets
- Superior performance with long (up to 500 bp) and GC-rich templates
- UNG treatment capability due to combination of dUTP/dTTP
- Easy pipetting due to blue visualisation dye

Composition:
BGTAQ HF/HT HotTaq DNA polymerase
qPCR buffer
5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
dNTPs, including dUTP/dTTP
EvaGreen® dyeInternal reference (based on ROX dye)
GC-enhancer
Visualisation dye

Shipping and Storage conditions:
Routine storage: -20ºC

Shipping at room temperature has no detrimental effects on the quality of this reagent.