BGREEN HotTaq EvaGreen qPCR Mix with ROX for ABI (1 mL, 250 rxn)

BGTAQ HF/HT HotTaq EvaGreen® qPCR Mix is optimized for real-time quantitative PCR assays.
The mix includes BGTAQ HF HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye (and ROX dye according to system requirements). Only template primers and water need to be added.
BGTAQ HF/HT HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C.
This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
BGTAQ HF/HT HotTaq EvaGreen® qPCR Mix is available with ROX or no ROX dye.

APPLICATIONS:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

Cycler compatibility:
BGTAQ HF/HT HotTaq EvaGreen® qPCR Mix (ROX) can be used with all major qPCR cyclers requiring high ROX dye level, including:
ABI PRISM® 5700, 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block)
Stratagene Mx3000P™, Mx3005P™ and Mx4000®

Composition:
BGTAQ HF/HT HotTaq DNA polymerase
5x qPCR buffer E
12.5 mM MgCl2: 1 x PCR solution – 2.5 mM MgCl2
dNTPs, including dTTP to improve reaction sensitivity and efficiency
EvaGreen®dye*
ROX dye**

Storage conditions
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of BGTAQ HF/HT HotTaq EvaGreen® qPCR Mix.

* EvaGreen® is a DNA-binding dye for qPCR that, compared to SYBR® Green I, has similar spectra but much less PCR inhibition. It is extremely stable and nonmutagenic/ noncytotoxic. EvaGreen® is compatible with all common qPCR cyclers by selecting the standard settings for SYBR® Green or FAM.
"EvaGreen® is a registered trademark of BIOTIUM, INC."

"SYBR® is a registered trademark of Molecular Probes, Inc."

**ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

This product is supplied for research use only.