BGREEN HotTaq EvaGreen qPCR HRM Mix with ROX (1 mL, 250 rxn)

BGTAQ HF/HT HotTaq EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: BGTAQ HF/HT HotTaq DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. Only water, template and primers need to be added.
BGTAQ HF/HT HotTaq DNA Polymerase is activated by a 12 min incubation step at 95°C.
This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
BGTAQ HF/HT HotTaq EvaGreen® HRM Mix is available with ROX or no ROX dye.

APPLICATIONS:
High Resolution Melt (HRM)

Composition:
BGTAQ HF/HT HotTaq DNA polymerase
5 x EvaGreen®HRM buffer
dNTPs
5 mM MgCl2 1 x PCR solution – 2.5 mM MgCl2
EvaGreen® dye*
BSA
ROX dye**

Storage conditions:
Routine storage: -20ºC

Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of BGTAQ HF/HT HotTaq EvaGreen® HRM Mix.

* EvaGreen® is a DNA-binding dye for qPCR that, compared to SYBR® Green I, has similar spectra but much less PCR inhibition. It is extremely stable and nonmutagenic/ noncytotoxic. EvaGreen® is compatible with all common qPCR cyclers by selecting the standard settings for SYBR® Green or FAM.

"EvaGreen® is a registered trademark of BIOTIUM, INC."

**ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.