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BGREEN HotTaq EvaGreen qPCR HRM Mix NO ROX (1 mL, 250 rxn)
Cod. art.: | 0400301E |
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Catalogo: | Biologia molecolare |
Categoria: | qPCR Mixes |
Tipologia: | Real-time PCR |
Formato: | qPCR HRM NO ROX |
Temperatura: | -20°C |
Marca: | Biogenerica |
MePA: | Richiedi integrazione mepa@biogenerica.it |
Quantità: |
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Descrizione
BGTAQ HF/HT HotTaq EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: BGTAQ HF/HT HotTaq DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. Only water, template and primers need to be added.
BGTAQ HF/HT HotTaq DNA Polymerase is activated by a 12 min incubation step at 95°C.
This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
BGTAQ HF/HT HotTaq EvaGreen® HRM Mix is available with ROX or no ROX dye.
APPLICATIONS:
High Resolution Melt (HRM)
Composition:
BGTAQ HF/HT HotTaq DNA polymerase
5 x EvaGreen®HRM buffer
dNTPs
5 mM MgCl2 1 x PCR solution – 2.5 mM MgCl2
EvaGreen® dye*
BSA
NO ROX dye**
Storage conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of BGTAQ HF/HT HotTaq EvaGreen® HRM Mix.
* EvaGreen® is a DNA-binding dye for qPCR that, compared to SYBR® Green I, has similar spectra but much less PCR inhibition. It is extremely stable and nonmutagenic/ noncytotoxic. EvaGreen® is compatible with all common qPCR cyclers by selecting the standard settings for SYBR® Green or FAM.
"EvaGreen® is a registered trademark of BIOTIUM, INC."
**ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.