BGP HotTaq Probe qPCR Universal Mix (1 mL, 250 rxn)

BGTAQ HF/HTHotTaq Probe qPCR Universal Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform singleplex or duplex qPCR, with the exception of template, primers, and probes.
The qPCR Mix contains optimized components and BGTAQ HF/HS HotTaq DNA polymerase supplied in a proprietary reaction buffer that enables efficient amplification of regular and GC-rich targets. BGTAQ HF/HT HotTaq Probe qPCR Universal Mix is optimized for DNA/LNA hydrolysis probes based on the 5' flap endonuclease activity. BGTAQ HF/HS HotTaq DNA polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

APPLICATIONS:
- DNA/LNA hydrolysis probe-based assays
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

Composition:
BGTAQ HF/HT HotTaq DNA polymerase
BGTAQ HF/HS HotTaqProbe qPCR Universal buffer
15 mM MgCl2 1x PCR solution – 3 mM MgCl2
dNTPs, including dUTP. The mix allows UNG treatment to prevent carryover contamination from previous runs.
In a separate vial: 100% DMSO is included in the kit in a separate vial.

IMPORTANT: UNG is not included in the BGTAQ HF/HS HotTaq Probe qPCR Universal Mix and must be purchased separately.

Internal reference based on ROX dye*.

For multiplex application: if ROX dye is used as one of the fluorophores, internal reference might interfere with the signal – a version without ROX is available upon request.

*ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Storage conditions:
Routine storage: -20ºC.
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of BGTAQ HF/HS HotTaq Probe qPCR Universal Mix.