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BGP HotTaq Probe qPCR Mix with ROX for ABI (1 mL, 250 rxn)
Cod. art.: | 0400311E |
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Catalogo: | Biologia molecolare |
Categoria: | qPCR Mixes |
Tipologia: | Real-time PCR |
Formato: | qPCR probe ROX |
Temperatura: | -20°C |
Marca: | Biogenerica |
MePA: | Richiedi integrazione mepa@biogenerica.it |
Quantità: |
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Descrizione
BGTAQ HF/HT HotTaq Probe qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and BGTAQ HF/HT HotTaq DNA polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
BGTAQ HF/HT HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
BGTAQ HF/HT HotTaq Probe qPCR Mix is available with ROX or no ROX dye.
APPLICATIONS:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
Cycler and Probe compatibility:
BGTAQ HF/HT HotTaq Probe qPCR Mix (ROX) is compatible for use with any probe system and qPCR cyclers requiring high ROX dye level, including :
ABI PRISM® 5700, 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block)
Stratagene Mx3000P™, Mx3005P™ and Mx4000®
Composition:
BGTAQ HF/HT HotTaq DNA polymerase
5x qPCR buffer P
15 mM MgCl2: 1 x PCR solution – 3 mM MgCl2
dNTPs, including dTTP to improve reaction sensitivity and efficiency
ROX dye*
*ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.
Storage conditions:
Routine storage: -20ºC. Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of BGTAQ HF/HT HotTaq Probe qPCR Mix.
This product is supplied for research use only.