BGP HotTaq Probe qPCR Mix NO ROX for Bio-rad, Rotor-Gene, Mastercycler (1 mL, 250 rxn)

BGTAQ HF/HT HotTaq Probe qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and BGTAQ HF/HT HotTaq DNA polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
BGTAQ HF/HT HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
BGTAQ HF/HT HotTaq Probe qPCR Mix is available with ROX or no ROX dye.

APPLICATIONS:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

Cycler and Probe compatibility:
BGTAQ HF/HT HotTaq Probe qPCR Mix (ROX) is compatible for use with any probe system and qPCR cyclers that do not require reference dye, including
Bio-Rad iQ™ 5, Opticon™, Opticon™ 2; Chromo 4™, MiniOpticon, CFX96, CFX384
Cepheid SmartCycler™
Corbett Rotor-Gene™ 3000
Rotor-Gene™ 6000
Eppendorf Mastercycler® ep realplex
Roche LightCycler® 480
Techne Quantica™

Composition:
BGTAQ HF/HT HotTaq DNA polymerase
5x qPCR buffer P
15 mM MgCl2: 1 x PCR solution – 3 mM MgCl2
dNTPs, including dTTP to improve reaction sensitivity and efficiency
NO ROX dye*

*ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Storage conditions:
Routine storage: -20ºC. Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of BGTAQ HF/HT HotTaq Probe qPCR Mix.

This product is supplied for research use only.