Precision Plus Protein WesternC Blotting Standards, value pack, (250 µl)

Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development.
Product Contents
1 - Precision Plus Protein WesternC Standards, 250 µl (1610376)
1 - Precision Protein™ StrepTactin-HRP Conjugate, 125 µl (1610381)
Applications and Uses

Monitoring electrophoretic separation
Monitoring blot transfer efficiency with easy orientation via multicolor standards
Molecular weight sizing of proteins after blot development
Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range
More Information
Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue.
Fluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. No conjugate is needed for fluorescent blot detection.

Packaging Options
Number of ApplicationsCatalog Number
501610385
2501610398

MW size range, kD10–250
Stain typeCombination of stained Dual Color and unstained
Volume, µlN/A
Total number of applications50
Shelf life at –20°C, monthsfor WesternC standards, 12
Shelf life at 4°C, monthsfor StrepTactin-HRP conjugate, 12
Loading volume on mini gels, µlfor chemiluminescent detection, 5
Loading volume on mini gels, µlfor fluorescent blot detection, 5 of 1:10 dilution; StrepTactin conjugate is not needed for fluorescent blotting applications
Application Type
Monitoring electrophoretic separation for SDS-PAGE gelsYes
Molecular weight sizing on SDS-PAGE gelsYes
Monitoring blot transfer efficiencyYes
Fluorescence blotsYes
Molecular weight sizing onBlots
Loading Volume on Mini Gels
For Coomassie staining, µlN/A
For stain-free gels, µlN/A
For silver staining, µlN/A
For SYPRO Ruby staining, µlN/A
For blot development, µlN/A
StrepTactin conjugate working dilutions for colorimetric blot developmentN/A
StrepTactin conjugate working dilutions for chemiluminescence blot developmentN/A