BGTAQ PFU DNA Polymerase (2500 Units)

In addition to 5 ´ to 3 ´ DNA polymerase activity, BGTAQ Pfu DNA Polymerase also possesses 3 ´ to 5 ´ exonuclease (proofreading) activity. BGTAQ Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to ten fold more accurate than normal Taq DNA polymerase.
Consequently, BGTAQ Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.

APPLICATIONS:
- Close to ten times more accurate than normal DNA polymerase
- Produces blunt-ended amplification products to be used for cloning
- Remains active even after incubating 90 minutes at 90°C

SOURCE:
Recombinant E. coli strain with cloned gene encoding Pyrococcus furiosus DNA polymerase.
UNIT DEFINITION:
One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 20 min at 70°C.
QUALITY CONTROL:
Free of detectable, non-specific nucleases.
STORAGE & DILUTION BUFFER:
40 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.5 mM EDTA, 150 mM KCl, 0.6% Nonidet P40, 0.7% Tween 20 and 40% glycerol.
CONCENTRATION:
2 units/µl

SUPPLIED WITH:
10x Pfu Buffer: 150 mM Tris-HCl (pH 8.8 at 25°C), 150 mM KCl, 150 mM (NH4)2SO4, 1% Triton X-100, 2 mg/ml BSA
10x Pfu Buffer with MgSO4: 250 mM Tris-HCl (pH 8.8 at 25°C), 150 mM KCl, 150 mM (NH4)2SO4, 1% Triton X-100, 2 mg/ml BSA, 30 mM MgSO4.
30 mM MgSO4
SHIPPING & STORAGE CONDITIONS:
Store at -20°C. Guaranteed stable for 1 year if properly stored.

This product is supplied for research use only.

Available in size of 500 Units, 1000 Units, 2500 Units or bulk.