BGTAQ HotStart DNA Polymerase (500 Units)

BGTAQ HT HotTaq DNA Polymerase catalyzes 5 ´ to 3 ´ synthesis of DNA, but lacks 3 ´ to 5 ´ exonuclease activity
Prior the first PCR step the BGTAQ HT HotTaq DNA Polymerase should be activated by 15 minute incubation at 95 - 97°C.

APPLICATIONS:
- Polymerase chain reaction setup at room temperature
- Effective incorporation of modified nucleotides

SOURCE:
Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.
UNIT DEFINITION:
One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.
QUALITY CONTROL:
Free of detectable, non-specific nucleases. Activity and stability tested at 20, 30 and 40 cycles of PCR reaction at 95°C. Tested for the absence of human DNA contamination by PCR with Alu-specific primers.
STORAGE & DILUTION BUFFER:
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
CONCENTRATION:
10 units/µl

SUPPLIED WITH:
10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
10x Taq Buffer with (NH4)2SO4: 750 mM Tris-HCl (pH 8.8 at 25°C),
200 mM (NH4)2SO4, 0.1% Tween 20.
25 mM MgCl2
SHIPPING AND STORAGE CONDITIONS:
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol
Store at -20°C.
Guaranteed stable for 12 months when properly stored

Comments: This product is supplied for research use only.

Available in size of 500 Units, 1000 Units, 2500 Units or bulk.