BGTAQ HFS highly processive HotStart DNA Polymerase (1000 Units)

BGTAQ HF/HT HotTaq DNA Polymerase is a chemically modified BGTAQ HF Taq DNA Polymerase.
At ambient temperatures it is inactive, having no polymerization activity.
BGTAQ HF/HT HotTaq DNA Polymerase is activated by a 15 min incubation step at 95°C.
This prevents extentension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup.
The enzyme has 5’>3’ polymerization-dependent exonuclease replacement activity but lacks 3’> 5’ exonuclease activity.

APPLICATIONS:
- Hot Start PCR
- Primer extension
- TA cloning

SOURCE:
Purified from an E. cUNIT DEFINITION:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 min at 74°C.
QUALITY CONTROL:
The enzyme is free of nicking and priming activities, exonucleases and unspecific endonucleases. SDS/PAGE - 95 kD band, >98% pure. Activity and stability tested via thermocycling. The error rate per nucleotide per cycle is ~2.5 x 105; the accuracy is ~4 x 104.
Estimated half life at 95°C is 1.5 hours.
STORAGE AND DILUTION BUFFER:
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.oli strain carrying an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase
CONCENTRATION:
5 units/µl

SUPPLIED WITH:
10x Reaction Buffer B1 (Mg2+, detergent free): Tris-HCl and (NH4)2SO4, 10x Reaction Buffer B2 (Mg2+ free): Tris-HCl, (NH4)2SO4 and detergent, 25 mM MgCl2, 10x Enhancer. Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution). Enhancer is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.
SHIPPING AND STORAGE CONDITIONS:
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this reagent.
Store at -20°C. Guaranteed stable for 1 year if properly stored.

This product is supplied for research use only.

Available in size of 500 Units, 1000 Units or bulk.