BGTAQ HF highly processive DNA Polymerase (500 Units)

BGTAQ HF is a highly processive, thermostable DNA polymerase. Due to its genetic modifications
BGTAQ HF has an enhanced stability at room temperature with no activity loss for up to 1 month
The enzyme has 5’>3’ polymerization-dependent exonuclease replacement activity but lacks 3’>5’
exonuclease activity.

APPLICATIONS:
- Suited for a wide range of PCR assays
- Primer extension.
- TA cloning

SOURCE:
Purified from an E. coli strain carrying an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
UNIT DEFINITION:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 min at 74°C.
QUALITY CONTROL:
The enzyme is free of nicking and priming activities, exonucleases and unspecific endonucleases. SDS/PAGE - 95 kD band, >98% pure. Activity and stability tested via thermocycling. The error rate per nucleotide per cycle is ~2.5 x 105; the accuracy is ~4 x 104.
Estimated half life at 95°C is 1.5 hours.
STORAGE AND DILUTION BUFFER:
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
CONCENTRATION:
5 units/µl

SUPPLIED WITH:
10x Reaction Buffer 1 (Mg2+ free): 800 mM Tris-HCl (pH 9.4 at 25°C), 200 mM (NH4)2SO4, 0.2% w/v Tween-20.
25 mM MgCl2
SHIPPING AND STORAGE CONDITIONS:
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this reagent.
Store at -20°C. Guaranteed stable for 1 year if properly stored.

This product is supplied for research use only.

Available in size of 500 Units, 1000 Units, 2000 Units or bulk.