BGTAQ DNA Polymerase, recombinant (500 Units)

BGTAQ DNA Polymerase is highly thermostable DNA polymerase
The enzyme catalyzes 5’ to 3’ synthesis of DNA, but lacks 3’ to 5’ exonuclease activity.

APPLICATIONS:
- Polymerase chain reaction (PCR)
- Routine PCR applications
- Primer extension
- DNA sequencing

SOURCE:
Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.
UNIT DEFINITION:
One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.
QUALITY CONTROL:
Free of detectable, non-specific nucleases.
STORAGE & DILUTION BUFFER:
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
CONCENTRATION:
5 units/µl
SUPPLIED WITH:
10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
10x Taq Buffer with (NH4)2SO4: 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH4)2SO4, 0.1% Tween 20. 25 mM MgCl2
SHIPPING & STORAGE CONDITIONS:
Store at -20°C. Guaranteed stable for 1 year if properly stored.
COMMENTS:
This product is supplied for research use only

Available in size of 500 Units, 1000 Units, 2500 Units or bulk.