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BGTAQ DNA Polymerase (1000 Units)
Cod. art.: | 0400111E |
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Catalogo: | Biologia molecolare |
Categoria: | DNA polymerases |
Tipologia: | Standard PCR |
Formato: | Separate buffers |
Temperatura: | -20°C |
Marca: | Biogenerica |
MePA: | Richiedi integrazione mepa@biogenerica.it |
Quantità: |
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Descrizione
BGTAQ DNA Polymerase is highly thermostable DNA polymerase
The enzyme catalyzes 5’ to 3’ synthesis of DNA, but lacks 3’ to 5’ exonuclease activity.
APPLICATIONS:
- Polymerase chain reaction (PCR)
- Routine PCR applications
- Primer extension
- DNA sequencing
SOURCE:
Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.
UNIT DEFINITION:
One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.
QUALITY CONTROL:
Free of detectable, non-specific nucleases.
STORAGE & DILUTION BUFFER:
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
CONCENTRATION:
5 units/µl
SUPPLIED WITH:
10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
10x Taq Buffer with (NH4)2SO4: 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH4)2SO4, 0.1% Tween 20. 25 mM MgCl2
SHIPPING & STORAGE CONDITIONS:
Store at -20°C. Guaranteed stable for 1 year if properly stored.
COMMENTS:
This product is supplied for research use only
Available in size of 500 Units, 1000 Units, 2500 Units or bulk.