InviTrap Spin Universal RNA Mini Kit (250 preps)

Downstream application
PCR techniques, NGS and hybridization methods
Target nucleic acid
RNA
Starting material
Cell culture: up to 1 x 107 cells/ml
Up to 1.5 ml fresh whole blood (EDTA / citrate stabilized, but not heparin)
Up to 20 mg fresh or frozen tissue
Yield
From cell culture: up to 80 µg total RNA e.g., 10 - 15 µg total RNA from
5 x 105 NIH 3T3 fibroblasts
From blood: up to 1 - 5 µg total RNA / ml of whole blood (depending on leukocyte count)
From tissue: up to 80 µg total RNA (depending on type and amount of starting material)
Preparation time
Cells: approx. 20 - 25 min (incl. lysis)
Blood: approx. 45 min (incl. Buffer EL treatment)
Tissue: approx. 15 - 20 min (excl. sample homogenization)
Elution Volume
30 – 100 µl
Technology
Spin column
Certification
CE-IVD (in compliance with REGULATION (EU) 2017/746 on in vitro diagnostic medical devices)