Invisorb Fragment CleanUp PCR and DNA fragments from gels (250 preps)

Downstream application
Sequencing, cloning, ligation, restriction enzymes digestion, PCR
Target nucleic acid
DNA
Starting material
up to 100 µl reaction volume:
PCR reaction mixture
restriction digestion mixture
ligation mixture
cDNA synthesis mixture
cycle sequencing reaction mixture
up to 300 mg of gel slices (0.8 – 2%) from TAE or TBE agarose gels
Recovery80 – 95 %, depends on fragment length (PCR clean-up)
60 - 90 %, depends on fragment length and type of agarose gel
Size range
Fragment sizes of 80 bp – 30 kb can be purified
Preparation time
approx. 7 min (PCR clean-up)
approx. 20 min (agarose gel extraction)
Elution Volume
10 - 50 µl
Technology
Spin column
Certification
RUO