BGM-MLV H-Minus Reverse Transcriptase (10000 Units)

BG H Minus M-MLV Reverse Transcriptase is a genetically modified M-MLV RT which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products.
The enzyme has RNA polymerizationdependent and DNA polymerization-dependent activity but lacks ribonuclease H activity.

APPLICATIONS:
- cDNA synthesis
- RNA analysis by primer extension
- DNA labeling

UNIT DEFINITION:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 370C.
QUALITY CONTROL:
Free of endo- and exodeoxyribonucleases, phosphatases and ribonuclease. Activity and stability tested in first strand cDNA synthesis.
STORAGE AND DILUTION BUFFER:
50% glycerol, 20 mM Tris-HCl pH 7.6 at 25oC, 150 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% IGEPAL CA-630
CONCENTRATION:
200 units/µl

SUPPLIED WITH:
Reaction Buffer with DTT: 250 mM Tris-HCl, 150 mM KCl, 40 mM MgCl2, 25 mM DTT 5X Reaction Buffer: 250 mM Tris-HCl, 150 mM KCl, 40 mM MgCl2, 100 mM DTT
SHIPPING AND STORAGE CONDITIONS:
Store at -20°C. Guaranteed stable for 1 year if properly stored.

This product is supplied for research use only.