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Illustra Plasmid Mini (50 pz)
Cod. art.: | 28904269 |
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Catalogo: | Proteomica |
Categoria: | Illustra |
Tipologia: | Plasmid Mini |
Temperatura: | RT |
Marca: | GE Amersham |
MePA: | Richiedi integrazione mepa@biogenerica.it |
Quantità: |
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Descrizione
The illustra plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification. Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane.
Total preparation time of less than 10 minutesrepresenting a 50% reduction in protocol time
Produces high-quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing
Increased ease of use provided minimal changes in pipetting volume from one part of the protocol to the next, less centrifugation steps, color-coded kit components, and lack of organic solvents
The typical plasmid yields from a fresh 1.5 ml culture of E.coli containing a high copy number plasmid is approximately 9 µg
Can accommodate high and low copy number plasmid purification.