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Agarose D5 standard, high gel strength for pulsed field technique (100 g)

Agarose D5 standard, high gel strength for pulsed field technique (100 g)
Cod. art.: 8045-100
Catalogo: Elettroforesi
Categoria: Agarosi standard per elettroforesi
Tipologia: pulsed field
Formato: 100 g
Temperatura: RT
Marca: Conda
MePA: Richiedi integrazione mepa@biogenerica.it
Quantità:

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Descrizione

D-5 Agarose is a linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses.
This characteristic, added to the very low sulfate content, produces an strong intercatenary interaction, yielding a gel with very
high gel strength and higher exclusion limit.

Features
Extremely high gel strength allowing for lower gel concentrations (0.3%), enabling it to be used not only with high molecular weight
nucleic acids, including chromosomes, but also with large sized particles like viruses and ribosomes.
High electrophoretic mobility. DNA mobility is greater when compared with D-1LE. Electrophoresis times are reduced
depending upon buffer and agarose concentration used.
Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
Greater thermal stability due to high hysteresis (difference between gelling and melting temperatures).
Exceptionally low absorption of staining agents.
Absence of toxicity (the alternative is polyacrylamide which can be toxic)

Applications
Conventional Electrophoresis: can be used in a wide range of concentrations
Pulsed Field Gel Electrophoresis: because of its higher exclusion limit, larger molecules can be separated
Blotting.
Agarose Beads preparation
Cell and enzyme immobilization

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