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Total RNA Purification Micro Kit. (50 preps)

Total RNA Purification Micro Kit. (50 preps)
Total RNA Purification Micro Kit. (50 preps)Total RNA Purification Micro Kit. (50 preps)
Cod. art.: 35300
Catalogo: Biologia molecolare
Marca: Norgen Biotek
Sul MePA: Richiedi integrazione mepa@biogenerica.it - Tel. 095 914678
Disponibilità: Spedizione stimata in 5/10 giorni. Per informazioni chiama lo 095914678
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Descrizione

Rapid purification of total RNA -including microRNA - from small input amounts

- Isolate true total RNA including siRNA and microRNA from small input amounts
- Small elution volume of 20 µL
- No phenol or chloroform extractions
- Isolate high quality RNA from a variety of sources, including LCM
- Extract RNA from as little as a single cell
Norgen’s Total RNA Purification Micro Kit provides a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, tissue samples, and microdissected samples including laser-capture microdissection (LCM). The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The special design of the micro spin-column allows a small elution volume of as little as 20 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Briefly, the cells or tissue of interest are first lysed using the provided Lysis Solution, ethanol is added and the RNA is bound to Norgen's column (BIND). Under these conditions only the RNA will bind to Norgen's resin while most of the contaminating cellular proteins are removed in the flowthrough or retained on top of the resin. The bound RNA is then washed to remove any remaining impurities (WASH). Lastly, the purified total RNA is eluted into 20 µL of the provided Elution Buffer or water (ELUTE). Please see procedure flowchart to the right.
Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing.

FEATURES AND BENEFITS

Ideal for small inputs - Total RNA can be isolated from small input amounts of cultured animal cells, tissue samples and microdissected samples including LCM. RNA has been isolated from as little as a single cell.
Smal elution volume - Elute total RNA in as little as 20 µL of Elution Buffer.
No phenol:chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform.
Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA) without compromising yield.
Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes.

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