Precision Plus Protein WesternC Blotting Standards, (250 µl)

Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development.
Applications and Uses

Monitoring electrophoretic separation
Monitoring blot transfer efficiency with easy orientation via multicolor standards
Molecular weight sizing of proteins after blot development
Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range
Packaging Options
Number of Applications (Volume)
Catalog Number
50 (250 µl)1610376
250 (5 x 250 µl)1610399

MW size range, kD10–250
Stain typeCombination of stained Dual Color and unstained
Volume, µl250
Total number of applications50
Shelf life at –20°C, months12
Loading volume on mini gels, µlfor chemiluminescent detection, 5. Precision Protein™ StrepTactin-HRP or -AP conjugate needed.
Loading volume on mini gels, µlfor fluorescent blot detection, 5 of 1:10 dilution
Application Type
Monitoring electrophoretic separation for SDS-PAGE gelsYes
Molecular weight sizing on SDS-PAGE gelsYes
Monitoring blot transfer efficiencyYes
Fluorescence blotsYes
Molecular weight sizing onBlots
Loading Volume on Mini Gels
For Coomassie staining, µlN/A
For stain-free gels, µlN/A
For silver staining, µlN/A
For SYPRO Ruby staining, µlN/A
For blot development, µlN/A
StrepTactin conjugate working dilutions for colorimetric blot developmentN/A
StrepTactin conjugate working dilutions for chemiluminescence blot developmentN/A