Flamingo Fluorescent Protein Gel Stain (100 mL)

Choose economical, sensitive Flamingo Fluorescent Protein Gel Stain for the detection of denatured proteins in gels including first-dimension isoelectric focusing (IEF) gels. The dye is non-fluorescent in solution and becomes strongly fluorescent on binding to protein.
Linear response over 3 orders of magnitude, with a lower limit of 0.25–0.5 ng
No destaining required, and gels cannot be overstained
Low background; readily distinguish spots and speckles
Stain may be exposed to typical room light for up to 8 hours without affecting the fluorescence intensity of stained protein bands or spots
Stained gels may be stored in the dark at 2–8°C for up to 6 months without significant loss of imaging sensitivity
Flamingo Fluorescent Protein Gel Stain can be visualized with laser-based gel imaging systems or UV detectors (Ex 512 nm, Em 535 nm, secondary Ex 271 nm; see Specifications tab for laser/emission filter compatibility). The staining procedure is a simple two-step protocol that can be completed in as little as 5 hours. The steps are not time-sensitive. Stained proteins are compatible with mass spectrometry and enzymatic digestion.

Packaging Options
Flamingo Fluorescent Protein Gel Stain, VolumeCatalog Number
20 ml1610490
100 ml1610491
500 ml1610492

Compatible laser/emission filterBlue laser 473 nm/530 nm
Blue laser 488 nm/560 nm LP
Green laser 532 nm/555 nm LP
UV transilluminatorExcitation centered on 300 nm
StorageStable for at least 6 months when stored at 2–8°C, protected from light