Descrizione

for Roche LightCycler® 480 platform, Bio-Rad Opticon, Opticon 2, Chromo4, MiniOpticon, CFX96, CFX384, Cepheid SmartCycler®, Qiagen Rotor-Gene 3000, 6000, Eppendorf Mastercycler® Ep realplex, Illumina® Eco, Takara Thermal Cycler Dice® (TP800) and Techne Quantica®

Features and Benefits

• Rapid - hot-start capability saves time and reduces primer-dimer formation
• Unique buffer chemistry - for highest specificity and sensitivity
• Sensitive – unique buffer chemistry for earlier Ct detection
• Simple and reproducible - just add primers and template

Instrument Compatibility
Roche LightCycler® 480 platform, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler®ep realplex, Illumina® Eco™, Takara Thermal Cycler Dice® (TP800) and Techne Quantica®

Description

The SensiFAST SYBR No-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments. A combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensures that the SensiFAST SYBR No-ROX Kit produces fast, highly-specific and ultra-sensitive real-time PCR.

Volume

BIO-98001: 200 x 20µl Reactions: 2 x 1ml
BIO-98005: 500 x 20µl Reactions: 5 x 1ml
BIO-98020: 2000 x 20µl Reactions: 20 x 1ml

Components

2x Reaction mix consisting of:
• Heat-activated DNA polymerase
• Ultra-pure dNTPs
• MgCl2
• SYBR® Green I

Kit Size

The kit size is based on a 20µl final reaction volume.

Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.

Notes

SensiFAST is a trademark of Bioline Reagents Ltd.