Descrizione

for ABI 7500, 7500 FAST, ViiA7, Stratagene (Agilent) Mx4000, Mx3000P, Mx3005P

Features and Benefits

• Rapid - hot-start capability saves time and reduces primer-dimer formation
• Accurate quantification - from low copy targets
• Sensitive – unique buffer chemistry for earlier Ct detection
• Flexible - compatible with many standard and fast cycling instruments

Instrument Compatibility

ABI 7500, 7500 FAST, ViiA7™, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™, as well as other instruments that do not require the use of a passive reference.

Description

The SensiFAST SYBR Lo-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments. A combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensures that the SensiFAST SYBR Lo-ROX Kit produces fast, highly-specific and ultra-sensitive real-time PCR.

The SensiFAST SYBR Lo-ROX Kit contains premixed ROX for optional use.

Volume

BIO-94001: 250 x 20µl Reactions: 2 x 1ml
BIO-94005: 500 x 20µl Reactions: 5 x 1ml
BIO-94020: 2000 x 20µl Reactions: 20 x 1ml


Components

2x Reaction mix consisting of:
• Heat-activated DNA polymerase
• Ultra-pure dNTPs
• MgCl2
• ROX
• SYBR® Green I

Kit Size

The kit size is based on a 20µl final reaction volume.

Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.


Notes

SensiFAST is a trademark of Bioline Reagents Ltd.