Descrizione

for ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne™, StepOne™ Plus.

Features and Benefits

• Rapid - hot-start capability saves time and reduces primer-dimer formation
• Unique buffer chemistry - for highest specificity and sensitivity
• Sensitive – unique buffer chemistry for earlier Ct detection
• Simple and reproducible - just add primers and template

Instrument Compatibility

ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne™, StepOne™ Plus each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX

Description

The SensiFAST SYBR Hi-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments. A combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensures that the SensiFAST SYBR Hi-ROX Kit produces fast, highly-specific and ultra-sensitive real-time PCR.

The SensiFAST SYBR Hi-ROX Kit contains premixed ROX for optional use.

Volume

BIO-92001: 200 x 20µl Reactions: 2 x 1ml
BIO-92005: 500 x 20µl Reactions: 5 x 1ml
BIO-92020: 2000 x 20µl Reactions: 20 x 1ml

Components

2x Reaction mix consisting of:
• Heat-activated DNA polymerase
• Ultra-pure dNTPs
• MgCl2
• ROX
• SYBR® Green I

Kit Size

The kit size is based on a 20µl final reaction volume.

Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.

Notes

SensiFAST is a trademark of Bioline Reagents Ltd.