Descrizione

For the rapid preparation of high quality DNA from whole blood, plasma and serum

- Input volumes of 500 µL to 2 mL
- No phenol required for DNA isolation
- Extract DNA from whole blood, plasma and serum
- Fast and convenient spin column protocol

Norgen’s Blood Genomic DNA Isolation Midi Kit is designed for the rapid preparation of genomic DNA from 500 µL up to 2 mL of whole blood, plasma and serum.
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Briefly, the sample is lysed and the released genomic DNA is bound to Norgen's column in the presence of Binding Solution (BIND). Under these conditions only the genomic DNA is bound to Norgen's resin while most of the contaminating RNA and cellular proteinaceous components are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities (WASH). Lastly, the purified genomic DNA is eluted into 300 - 600 µL of the provided Elution Buffer or water (ELUTE). Please see the procedure flowchart to the right.
The Blood Genomic DNA Isolation Midi Kit allows for the isolation of genomic DNA from the blood of various species, including humans. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 12 preparations. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including PCR and Southern Blot analysis.

FEATURES AND BENEFITS

No Phenol-Chloroform extraction or alcohol precipitation - Isolate genomic DNA without the use of harmful chemicals.
Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 30 minutes.
Isolate genomic DNA from various inputs - Isolate DNA from various blood inputs including plasma, whole blood and serum.
Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA is fully compatible with restriction enzyme digestions, Southern Blot and PCR analysis.
High quality DNA with no RNA contamination - No contamination or degradation of genomic DNA isolated with the Blood Genomic DNA Isolation Midi Kit is observed.