Descrizione

For the rapid preparation of high quality DNA from whole blood

- Input volumes up to 500 µL
- No phenol required for DNA isolation
- Fast and convenient spin column protocol

Norgen’s Blood Genomic DNA Isolation Kit is designed for the rapid preparation of genomic DNA from up to 500 µL of whole blood.
Purification is based on spin column chromatography. Briefly, the sample is lysed and the released genomic DNA is bound to Norgen's column under optimized salt concentrations (BIND). Under these conditions only the genomic DNA is bound to Norgen's column while most of the contaminating RNA and cellular proteinaceous components are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities (WASH). Lastly, the purified genomic DNA is eluted into 200 µL of the provided Elution Buffer (ELUTE). Please see the procedure flowchart to the right.
The Blood Genomic DNA Isolation Kit allows for the isolation of genomic DNA from the blood of various species, including humans. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 50 preparations. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including PCR and Southern Blot analysis.

FEATURES AND BENEFITS

No Phenol-Chloroform extraction or alcohol precipitation - Isolate genomic DNA without the use of harmful chemicals.
Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 30 minutes.
Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA is fully compatible with restriction enzyme digestions, Southern Blot and PCR analysis.
High quality DNA with no RNA contamination - No contamination or degradation of genomic DNA isolated with the Blood Genomic DNA Isolation Kit is observed.