Descrizione

For the rapid and convenient isolation of fungal DNA from plant surfaces

- Rapid isolation of fungal DNA from plant surfaces
- No liquid nitrogen or physical disruption methods required
- Isolate high quality DNA for a wide range of downstream applications

Norgen’s Direct Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from the fungi found on the surface of symptomatic plant samples. The major benefit of this kit is that it does not require the use of physical homogenization steps to isolate the fungal DNA, which are often time consuming and tedious procedures that are not suitable methods for use in the analysis of large numbers of samples. Furthermore, in the area of pathogen diagnosis simple and convenient sample preparation steps are a critical factor in supporting high throughput analysis. Therefore, Norgen’s Direct Fungi DNA Isolation Kit adopts a simple and rapid sample preparation procedure that does not rely on physical homogenization steps to isolate sufficient amounts of high quality fungal DNA that can be used in sensitive downstream detection methods.
Purification is based on spin column chromatography using Norgen's proprietary resin as the separation matrix. Briefly, the surface fungus is extracted from the plant tissue of interest by shaking with a small volume of Collection Solution. The collected fungi are then lysed, and the released fungal DNA is bound to Norgen's column (BIND). Under these conditions only the DNA will bind to Norgen's resin while most of the contaminating RNA and cellular proteinaceous components are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities (WASH). Lastly, the purified DNA is eluted into 50 - 100 µL of the provided Elution Buffer or water (ELUTE). Please see procedure flowchart to the right.

FEATURES AND BENEFITS

No liquid nitrogen or physical disruption methods required - Rapidly isolate fungal DNA from the surface of symptomatic plant samples without any physical disruption methods.
Rapid and simple processing - Rapid spin-column format and no physical disruption allows for the processing of multiple samples in 60 minutes.
High quality fungal DNA - The purified DNA is of the highest quality and can be used in a number of sensitive downstream applications, including PCR.
Purified DNA can be use in a number of downstream applications - Purified fungal DNA can be used in a number of downstream applications including real time PCR, sequencing, Southern blotting and SNP analysis.