Standard agaroses for routine analysis of nucleic acid fragments
D-1 Agarose is available for different uses in 4 types: Low EEO (electroendosmosis), Medium EEO, High EEO. More reliable and easier handling: - extraordinary mechanical resistance - possibility of pore size variation in accordance with particle size to be separated by modifying gel concentration - easy gel preparation: simple dissolution in aqueous buffers by standard boiling or microwaving - greater thermal stability due to high hysteresis (difference between gelling and melting temperatures) - excellent gel transparency and visibility - exceptionally low absorption of staining agents - absence of toxicity (unlike polyacrylamide, which is toxic)
Separation range: > 1,000 bp Optimum gel concentration: 0.75%, 1% and 1.25% Gelling temperature (1.5%): 36 ± 1.5°C Melting temperature (1.5%): 88 ± 1.5°C Applications: suitable for nucleic acid electrophoresis, blotting and radial immunodiffusion of proteins Certified DNAse/RNAse free