Descrizione

- Consistent results
- Premium Taq polymerase suited to a wide range of applications
- Processes fragments of up to 5Kb
- Leaves ´A´ overhang

Applications

* Routine PCR applications
* TA cloning

Description
BIOTAQ is widely used by molecular biologists that have come to depend upon the robust performance of this reagent.

BIOTAQ is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates, and is the ideal choice for most assays. BIOTAQ is a robust preparation and consistently delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

BIOTAQ is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl2 is provided to allow reaction conditions to be adjusted to suit the template.

The specificity and performance of BIOTAQ can be further improved with the use of 2x PolyMate Additive which is designed for GC- or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

BIOTAQ DNA Polymerase is purified from Thermus aquaticus

Unit Definition
One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.

Concentration
5u/ul

Storage Conditions
BIOTAQ can be stored for 12 months at -20°C.